Single copy-sensitive electrochemical assay for circulating methylated DNA in clinical samples with ultrahigh specificity based on a sequential discrimination–amplification strategy† †Electronic supplementary information (ESI) available. See DOI: 10.1039/c7sc01035d Click here for additional data file.
نویسندگان
چکیده
a Key Laboratory of Biomedical Information Engineering of Education Ministry, School of Life Science and Technology, Xi’an Jiaotong University, Xianning West Road, Xi’an, Shaanxi 710049, P. R. China. bDepartment of Respiratory Medicine, The Second Affiliated Hospital of Medical College, Xi’an Jiaotong University, Xiwu Road, Xi’an, Shaanxi 710049, P. R. China. cDivision of Physical Biology, Bioimaging Center, Shanghai Synchrotron Radiation Facility, CAS Key Laboratory of Interfacial Physics and Technology, Shanghai Institute of Applied Physics, Chinese Academy of Sciences, Shanghai 201800, P. R. China.
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Simple electrochemical sensing of attomolar proteins using fabricated complexes with enhanced surface binding avidity† †Electronic supplementary information (ESI) available. See DOI: 10.1039/c5sc00891c Click here for additional data file.
Various strategies have been proposed for the detection of disease protein biomarkers; however, most methods are too expensive, cumbersome or limited in sensitivity for clinical use. Here, we report that a fabricated complex can be used as a powerful tool to detect trace proteins in complex samples. In this strategy, a DNA–protein complex that comprises of one target molecule and two or more de...
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عنوان ژورنال:
دوره 8 شماره
صفحات -
تاریخ انتشار 2017